Recent technological developments have made it possible to detect Oxygen Consumption Rate OCR in living cells during the course of an experiment in a long-term, high-throughput manner. This ability can offer fresh perspectives on how cells divide, metabolise, differentiate, and react to stress in dynamic environments.
This white paper offers a thorough overview of OCR normalisation techniques, including as flow cytometry, protein-based, DNA content, and microscopy methods. In order to account for factors and uncover significant differences from your data, it discusses both internal and external normalisation strategies. Your lab can improve the accuracy of OCR measurements and gain a better understanding of mitochondrial function and its role in both health and illness.
